The Krebs Institute is a multidisciplinary research centre with an overall theme of mechanistic biology. Mechanistic biology studies basic, life-governing, processes from the cellular to the atomic level. Such an approach requires a synergy of skills and expertise from across the scientific disciplines. At the heart of the Krebs is the mission to foster and facilitate research from fundamental biological, chemical and physical studies, through to translation into biotechnological and medical applications.

The Krebs Institute

The Krebs Institute was established at Sheffield University in 1988 as a multi-disciplinary, cross-departmental research arm to facilitate our programmes aimed at the greater understanding of biomolecular processes.

The members of the Institute are funded by grants from a wide variety of external bodies including BBSRC, EPSRC, MRC, the Wellcome Trust, EU, industry and the Royal Society.

The Krebs Institute is named after Sir Hans Krebs who, between 1935 and 1954, carried out his Nobel Prize winning biochemical experiments at Sheffield on the crucial metabolic cycle that bears his name.

Research Areas

An interdisciplinary approach to biology

Rapidly advancing technological developments have led to an explosion in genomic and biological data. To translate this information into predictive biology requires a full understanding of the molecular mechanisms underpinning life. The Krebs Institute has the ambitious aim of bridging this void in our knowledge, by harnessing complementary teams in a broad range of disciplines across the University to address crucial questions in biology.

To achieve this end, the Krebs Institute maintains, strengthens and stimulates collaborations between molecular, cellular and structural biologists, microbiologists, biochemists, chemists, physicists, theoreticians, information scientists and clinicians. The Institute is inclusive, driving science between whatever approaches are necessary to tackle complex and long-term problems.

The Annual Krebs Lecture

Tuesday 24 April 2018, 6pm Lecture Theatre 1, The Diamond, 32 Leavygreave Road

The increasing power of cryoEM for macromolecular structure determination

by Dr Richard Henderson, FRS FMedSci

Dr Richard Henderson is a molecular biologist and biophysicist, pioneer in the field of electron microscopy of biological molecules, and 2017 Nobel Prize winner in Chemistry. For further information on Dr Henderson’s Nobel Prize in Chemistry click on the link

Lecture synopsis: In the last few years, single particle electron cryomicroscopy (cryoEM) has experienced a quantum leap in its capability, due to improved electron microscopes, better detectors and better software, and this is revolutionising structural biology. Using the technique invented by Jacques Dubochet and his colleagues, a thin film containing a suspension of the macromolecules of interest is plunge-frozen into liquid ethane at liquid nitrogen temperature, creating a frozen sample in which individual images of the structures can be seen in many different orientations. Subsequent computer-based image analysis is then used to determine the three-dimensional structure, frequently at near-atomic resolution. I will describe some recent results and discuss remaining barriers to progress. CryoEM is already a very powerful method, but there are still many improvements that can be made before the approach reaches its theoretical

Book a place here

The Krebs Institute Symposium 2018

12th April 2018 | Lecture Theatre 3, The Diamond

9:15 Registration

9.30 Opening – Prof Jamie Hobbs, Director of the Krebs Institute.
9.40 Dr Nicola Green “New Methods for Biological Imaging”
10.10 Prof Ingunn Holen “Seeing is believing – Imaging cancer progression in vivo”
10.40 Dr William Wood “The dynamic photosynthetic membrane”
11.00 Coffee break
11.30 Prof Per Bullough “Biological Electron Microscopy in Sheffield – CryoEM and other facilities”
12.00 Prof Steve Matcher “Clinical biophotonic imaging”
12.30 Prof Julia Weinstein “Light and electron microscopy with transition metal complexes”

13.00 Lunch

13.50 Dr Rob Wilkinson “Modelling remodelling: investigating genetic control of blood vessel formation and function using light sheet imaging in zebrafish”
14.20 Prof Jim Wild “Hyperpolarised gas magnetic resonance imaging”
14.50 Flash presentations

15.30 Coffee break

16.00 Dr Pu Qian “Cryo-EM structure of reaction centre light harvesting complex from purple photosynthetic bacterium Blastochloris viridis”
16.20 Dr Ashley Cadby “A little background”
16:50 Dr Ahtasham Raza “Oxygen imaging and Photodynamic therapy of melanoma 3D model using metal complexes”

17.10 Close

KrebsFest

Exploring hidden worlds

The KrebsFest is a celebration of the scientific research of Sir Hans Krebs, winner of the Nobel Prize for Physiology or Medicine in 1953, for his pioneering work at the University of Sheffield.

At the festival we explore Krebs’ legacy through a series of public events and exhibitions including talks from Nobel Prize winners.

Find out more about KrebsFest at the festival website. 

Contact

Get in touch with us at the Krebs Institute

For further information, or to discuss collaborations, please contact the Director of the Krebs Institute:

Professor Jamie Hobbs

Email: jamie.hobbs@sheffield.ac.uk

Tel: +44 114 2224532

The Krebs Institute, MBB, The University of Sheffield, Sheffield S10 2TN, U.K.